mouse anti p38 Search Results


p38  (Miltenyi Biotec)
93
Miltenyi Biotec p38
PI3K/AKT signaling pathway is involved in the inhibitory effect of RES on NF-κB activation. ( A ) GICs (412) were treated with DMSO (vehicle) and 5 μM, 10 μM, and 20 μM RES for 48 h. Total expression and phosphorylation of Akt and mTOR were analyzed by western blotting using anti-Akt, anti-p-Akt, anti-mTOR, anti-p-mTOR, antibodies. β-actin was used as a loading control; ( B ) Total expression and phosphorylation of JNK, ERK, and <t>p38</t> were analyzed by western blotting using anti-JNK, anti-p-JNK, anti-ERK, anti-p-ERK, anti-p38 anti-p-p38 antibodies. β-actin was used as a loading control; ( C ) GICs (412) were pre-treated with IGF-1 (200 ng·mL −1 ) or wortmannin (5 μM) for 30 min and then incubated in the presence or absence of RES (10 μM). GICs were then subjected to cell invasion assay. Then, randomly chosen fields were photographed (200×), and the number of cells that migrated to the lower surface was calculated as a percentage of invasion. Data are shown as the mean ± SD of three independent experiments by Student’s t -test. * p < 0.05, ** p < 0.01; ( D ) GICs were pre-treated with IGF-1 (200 ng·mL −1 ) or wormannin (5 μM) for 30 min and incubated in the presence or absence of RES (10 μM) for 48 h, and then, the cell lysates were subjected to western blotting with anti-p-Akt and anti-Akt antibodies. β-actin was used as a loading control. The CM were subjected to gelatin zymography assay to determine the activity of MMP-2.
P38, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
p38 - by Bioz Stars, 2026-02
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p38α af8691 biotechne  (Bio-Techne corporation)
94
Bio-Techne corporation p38α af8691 biotechne
PI3K/AKT signaling pathway is involved in the inhibitory effect of RES on NF-κB activation. ( A ) GICs (412) were treated with DMSO (vehicle) and 5 μM, 10 μM, and 20 μM RES for 48 h. Total expression and phosphorylation of Akt and mTOR were analyzed by western blotting using anti-Akt, anti-p-Akt, anti-mTOR, anti-p-mTOR, antibodies. β-actin was used as a loading control; ( B ) Total expression and phosphorylation of JNK, ERK, and <t>p38</t> were analyzed by western blotting using anti-JNK, anti-p-JNK, anti-ERK, anti-p-ERK, anti-p38 anti-p-p38 antibodies. β-actin was used as a loading control; ( C ) GICs (412) were pre-treated with IGF-1 (200 ng·mL −1 ) or wortmannin (5 μM) for 30 min and then incubated in the presence or absence of RES (10 μM). GICs were then subjected to cell invasion assay. Then, randomly chosen fields were photographed (200×), and the number of cells that migrated to the lower surface was calculated as a percentage of invasion. Data are shown as the mean ± SD of three independent experiments by Student’s t -test. * p < 0.05, ** p < 0.01; ( D ) GICs were pre-treated with IGF-1 (200 ng·mL −1 ) or wormannin (5 μM) for 30 min and incubated in the presence or absence of RES (10 μM) for 48 h, and then, the cell lysates were subjected to western blotting with anti-p-Akt and anti-Akt antibodies. β-actin was used as a loading control. The CM were subjected to gelatin zymography assay to determine the activity of MMP-2.
P38α Af8691 Biotechne, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p38α af8691 biotechne/product/Bio-Techne corporation
Average 94 stars, based on 1 article reviews
p38α af8691 biotechne - by Bioz Stars, 2026-02
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mouse anti-p38 mapk monoclonal (612280)  (Becton Dickinson)
90
Becton Dickinson mouse anti-p38 mapk monoclonal (612280)
PI3K/AKT signaling pathway is involved in the inhibitory effect of RES on NF-κB activation. ( A ) GICs (412) were treated with DMSO (vehicle) and 5 μM, 10 μM, and 20 μM RES for 48 h. Total expression and phosphorylation of Akt and mTOR were analyzed by western blotting using anti-Akt, anti-p-Akt, anti-mTOR, anti-p-mTOR, antibodies. β-actin was used as a loading control; ( B ) Total expression and phosphorylation of JNK, ERK, and <t>p38</t> were analyzed by western blotting using anti-JNK, anti-p-JNK, anti-ERK, anti-p-ERK, anti-p38 anti-p-p38 antibodies. β-actin was used as a loading control; ( C ) GICs (412) were pre-treated with IGF-1 (200 ng·mL −1 ) or wortmannin (5 μM) for 30 min and then incubated in the presence or absence of RES (10 μM). GICs were then subjected to cell invasion assay. Then, randomly chosen fields were photographed (200×), and the number of cells that migrated to the lower surface was calculated as a percentage of invasion. Data are shown as the mean ± SD of three independent experiments by Student’s t -test. * p < 0.05, ** p < 0.01; ( D ) GICs were pre-treated with IGF-1 (200 ng·mL −1 ) or wormannin (5 μM) for 30 min and incubated in the presence or absence of RES (10 μM) for 48 h, and then, the cell lysates were subjected to western blotting with anti-p-Akt and anti-Akt antibodies. β-actin was used as a loading control. The CM were subjected to gelatin zymography assay to determine the activity of MMP-2.
Mouse Anti P38 Mapk Monoclonal (612280), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti-p38 mapk monoclonal (612280)/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
mouse anti-p38 mapk monoclonal (612280) - by Bioz Stars, 2026-02
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mouse anti-p-p38  (Beyotime)
90
Beyotime mouse anti-p-p38
PI3K/AKT signaling pathway is involved in the inhibitory effect of RES on NF-κB activation. ( A ) GICs (412) were treated with DMSO (vehicle) and 5 μM, 10 μM, and 20 μM RES for 48 h. Total expression and phosphorylation of Akt and mTOR were analyzed by western blotting using anti-Akt, anti-p-Akt, anti-mTOR, anti-p-mTOR, antibodies. β-actin was used as a loading control; ( B ) Total expression and phosphorylation of JNK, ERK, and <t>p38</t> were analyzed by western blotting using anti-JNK, anti-p-JNK, anti-ERK, anti-p-ERK, anti-p38 anti-p-p38 antibodies. β-actin was used as a loading control; ( C ) GICs (412) were pre-treated with IGF-1 (200 ng·mL −1 ) or wortmannin (5 μM) for 30 min and then incubated in the presence or absence of RES (10 μM). GICs were then subjected to cell invasion assay. Then, randomly chosen fields were photographed (200×), and the number of cells that migrated to the lower surface was calculated as a percentage of invasion. Data are shown as the mean ± SD of three independent experiments by Student’s t -test. * p < 0.05, ** p < 0.01; ( D ) GICs were pre-treated with IGF-1 (200 ng·mL −1 ) or wormannin (5 μM) for 30 min and incubated in the presence or absence of RES (10 μM) for 48 h, and then, the cell lysates were subjected to western blotting with anti-p-Akt and anti-Akt antibodies. β-actin was used as a loading control. The CM were subjected to gelatin zymography assay to determine the activity of MMP-2.
Mouse Anti P P38, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti-p-p38/product/Beyotime
Average 90 stars, based on 1 article reviews
mouse anti-p-p38 - by Bioz Stars, 2026-02
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mouse monoclonal anti-p-p38  (Promega)
90
Promega mouse monoclonal anti-p-p38
PI3K/AKT signaling pathway is involved in the inhibitory effect of RES on NF-κB activation. ( A ) GICs (412) were treated with DMSO (vehicle) and 5 μM, 10 μM, and 20 μM RES for 48 h. Total expression and phosphorylation of Akt and mTOR were analyzed by western blotting using anti-Akt, anti-p-Akt, anti-mTOR, anti-p-mTOR, antibodies. β-actin was used as a loading control; ( B ) Total expression and phosphorylation of JNK, ERK, and <t>p38</t> were analyzed by western blotting using anti-JNK, anti-p-JNK, anti-ERK, anti-p-ERK, anti-p38 anti-p-p38 antibodies. β-actin was used as a loading control; ( C ) GICs (412) were pre-treated with IGF-1 (200 ng·mL −1 ) or wortmannin (5 μM) for 30 min and then incubated in the presence or absence of RES (10 μM). GICs were then subjected to cell invasion assay. Then, randomly chosen fields were photographed (200×), and the number of cells that migrated to the lower surface was calculated as a percentage of invasion. Data are shown as the mean ± SD of three independent experiments by Student’s t -test. * p < 0.05, ** p < 0.01; ( D ) GICs were pre-treated with IGF-1 (200 ng·mL −1 ) or wormannin (5 μM) for 30 min and incubated in the presence or absence of RES (10 μM) for 48 h, and then, the cell lysates were subjected to western blotting with anti-p-Akt and anti-Akt antibodies. β-actin was used as a loading control. The CM were subjected to gelatin zymography assay to determine the activity of MMP-2.
Mouse Monoclonal Anti P P38, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal anti-p-p38/product/Promega
Average 90 stars, based on 1 article reviews
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anti-mouse p-p38  (CapitalBio Corporation)
90
CapitalBio Corporation anti-mouse p-p38
PI3K/AKT signaling pathway is involved in the inhibitory effect of RES on NF-κB activation. ( A ) GICs (412) were treated with DMSO (vehicle) and 5 μM, 10 μM, and 20 μM RES for 48 h. Total expression and phosphorylation of Akt and mTOR were analyzed by western blotting using anti-Akt, anti-p-Akt, anti-mTOR, anti-p-mTOR, antibodies. β-actin was used as a loading control; ( B ) Total expression and phosphorylation of JNK, ERK, and <t>p38</t> were analyzed by western blotting using anti-JNK, anti-p-JNK, anti-ERK, anti-p-ERK, anti-p38 anti-p-p38 antibodies. β-actin was used as a loading control; ( C ) GICs (412) were pre-treated with IGF-1 (200 ng·mL −1 ) or wortmannin (5 μM) for 30 min and then incubated in the presence or absence of RES (10 μM). GICs were then subjected to cell invasion assay. Then, randomly chosen fields were photographed (200×), and the number of cells that migrated to the lower surface was calculated as a percentage of invasion. Data are shown as the mean ± SD of three independent experiments by Student’s t -test. * p < 0.05, ** p < 0.01; ( D ) GICs were pre-treated with IGF-1 (200 ng·mL −1 ) or wormannin (5 μM) for 30 min and incubated in the presence or absence of RES (10 μM) for 48 h, and then, the cell lysates were subjected to western blotting with anti-p-Akt and anti-Akt antibodies. β-actin was used as a loading control. The CM were subjected to gelatin zymography assay to determine the activity of MMP-2.
Anti Mouse P P38, supplied by CapitalBio Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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mouse anti-β-actin antibody  (Amresco)
90
Amresco mouse anti-β-actin antibody
PI3K/AKT signaling pathway is involved in the inhibitory effect of RES on NF-κB activation. ( A ) GICs (412) were treated with DMSO (vehicle) and 5 μM, 10 μM, and 20 μM RES for 48 h. Total expression and phosphorylation of Akt and mTOR were analyzed by western blotting using anti-Akt, anti-p-Akt, anti-mTOR, anti-p-mTOR, antibodies. β-actin was used as a loading control; ( B ) Total expression and phosphorylation of JNK, ERK, and <t>p38</t> were analyzed by western blotting using anti-JNK, anti-p-JNK, anti-ERK, anti-p-ERK, anti-p38 anti-p-p38 antibodies. β-actin was used as a loading control; ( C ) GICs (412) were pre-treated with IGF-1 (200 ng·mL −1 ) or wortmannin (5 μM) for 30 min and then incubated in the presence or absence of RES (10 μM). GICs were then subjected to cell invasion assay. Then, randomly chosen fields were photographed (200×), and the number of cells that migrated to the lower surface was calculated as a percentage of invasion. Data are shown as the mean ± SD of three independent experiments by Student’s t -test. * p < 0.05, ** p < 0.01; ( D ) GICs were pre-treated with IGF-1 (200 ng·mL −1 ) or wormannin (5 μM) for 30 min and incubated in the presence or absence of RES (10 μM) for 48 h, and then, the cell lysates were subjected to western blotting with anti-p-Akt and anti-Akt antibodies. β-actin was used as a loading control. The CM were subjected to gelatin zymography assay to determine the activity of MMP-2.
Mouse Anti β Actin Antibody, supplied by Amresco, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
mouse anti-β-actin antibody - by Bioz Stars, 2026-02
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alexa fluor 647 mouse anti-human p38  (Becton Dickinson)
90
Becton Dickinson alexa fluor 647 mouse anti-human p38
PI3K/AKT signaling pathway is involved in the inhibitory effect of RES on NF-κB activation. ( A ) GICs (412) were treated with DMSO (vehicle) and 5 μM, 10 μM, and 20 μM RES for 48 h. Total expression and phosphorylation of Akt and mTOR were analyzed by western blotting using anti-Akt, anti-p-Akt, anti-mTOR, anti-p-mTOR, antibodies. β-actin was used as a loading control; ( B ) Total expression and phosphorylation of JNK, ERK, and <t>p38</t> were analyzed by western blotting using anti-JNK, anti-p-JNK, anti-ERK, anti-p-ERK, anti-p38 anti-p-p38 antibodies. β-actin was used as a loading control; ( C ) GICs (412) were pre-treated with IGF-1 (200 ng·mL −1 ) or wortmannin (5 μM) for 30 min and then incubated in the presence or absence of RES (10 μM). GICs were then subjected to cell invasion assay. Then, randomly chosen fields were photographed (200×), and the number of cells that migrated to the lower surface was calculated as a percentage of invasion. Data are shown as the mean ± SD of three independent experiments by Student’s t -test. * p < 0.05, ** p < 0.01; ( D ) GICs were pre-treated with IGF-1 (200 ng·mL −1 ) or wormannin (5 μM) for 30 min and incubated in the presence or absence of RES (10 μM) for 48 h, and then, the cell lysates were subjected to western blotting with anti-p-Akt and anti-Akt antibodies. β-actin was used as a loading control. The CM were subjected to gelatin zymography assay to determine the activity of MMP-2.
Alexa Fluor 647 Mouse Anti Human P38, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alexa fluor 647 mouse anti-human p38/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
alexa fluor 647 mouse anti-human p38 - by Bioz Stars, 2026-02
90/100 stars
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mouse monoclonal anti-p-p38 mapk (d-8; 1:1000) antibodies  (Abace Biotechnology Co Ltd)
90
Abace Biotechnology Co Ltd mouse monoclonal anti-p-p38 mapk (d-8; 1:1000) antibodies
PI3K/AKT signaling pathway is involved in the inhibitory effect of RES on NF-κB activation. ( A ) GICs (412) were treated with DMSO (vehicle) and 5 μM, 10 μM, and 20 μM RES for 48 h. Total expression and phosphorylation of Akt and mTOR were analyzed by western blotting using anti-Akt, anti-p-Akt, anti-mTOR, anti-p-mTOR, antibodies. β-actin was used as a loading control; ( B ) Total expression and phosphorylation of JNK, ERK, and <t>p38</t> were analyzed by western blotting using anti-JNK, anti-p-JNK, anti-ERK, anti-p-ERK, anti-p38 anti-p-p38 antibodies. β-actin was used as a loading control; ( C ) GICs (412) were pre-treated with IGF-1 (200 ng·mL −1 ) or wortmannin (5 μM) for 30 min and then incubated in the presence or absence of RES (10 μM). GICs were then subjected to cell invasion assay. Then, randomly chosen fields were photographed (200×), and the number of cells that migrated to the lower surface was calculated as a percentage of invasion. Data are shown as the mean ± SD of three independent experiments by Student’s t -test. * p < 0.05, ** p < 0.01; ( D ) GICs were pre-treated with IGF-1 (200 ng·mL −1 ) or wormannin (5 μM) for 30 min and incubated in the presence or absence of RES (10 μM) for 48 h, and then, the cell lysates were subjected to western blotting with anti-p-Akt and anti-Akt antibodies. β-actin was used as a loading control. The CM were subjected to gelatin zymography assay to determine the activity of MMP-2.
Mouse Monoclonal Anti P P38 Mapk (D 8; 1:1000) Antibodies, supplied by Abace Biotechnology Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal anti-p-p38 mapk (d-8; 1:1000) antibodies/product/Abace Biotechnology Co Ltd
Average 90 stars, based on 1 article reviews
mouse monoclonal anti-p-p38 mapk (d-8; 1:1000) antibodies - by Bioz Stars, 2026-02
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mouse anti-p38-b2 (number 33–8700 used at 5 mg/immunoprecipitation)  (Amersham Pharmacia Biotech Ltd)
90
Amersham Pharmacia Biotech Ltd mouse anti-p38-b2 (number 33–8700 used at 5 mg/immunoprecipitation)
PI3K/AKT signaling pathway is involved in the inhibitory effect of RES on NF-κB activation. ( A ) GICs (412) were treated with DMSO (vehicle) and 5 μM, 10 μM, and 20 μM RES for 48 h. Total expression and phosphorylation of Akt and mTOR were analyzed by western blotting using anti-Akt, anti-p-Akt, anti-mTOR, anti-p-mTOR, antibodies. β-actin was used as a loading control; ( B ) Total expression and phosphorylation of JNK, ERK, and <t>p38</t> were analyzed by western blotting using anti-JNK, anti-p-JNK, anti-ERK, anti-p-ERK, anti-p38 anti-p-p38 antibodies. β-actin was used as a loading control; ( C ) GICs (412) were pre-treated with IGF-1 (200 ng·mL −1 ) or wortmannin (5 μM) for 30 min and then incubated in the presence or absence of RES (10 μM). GICs were then subjected to cell invasion assay. Then, randomly chosen fields were photographed (200×), and the number of cells that migrated to the lower surface was calculated as a percentage of invasion. Data are shown as the mean ± SD of three independent experiments by Student’s t -test. * p < 0.05, ** p < 0.01; ( D ) GICs were pre-treated with IGF-1 (200 ng·mL −1 ) or wormannin (5 μM) for 30 min and incubated in the presence or absence of RES (10 μM) for 48 h, and then, the cell lysates were subjected to western blotting with anti-p-Akt and anti-Akt antibodies. β-actin was used as a loading control. The CM were subjected to gelatin zymography assay to determine the activity of MMP-2.
Mouse Anti P38 B2 (Number 33–8700 Used At 5 Mg/Immunoprecipitation), supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti-p38-b2 (number 33–8700 used at 5 mg/immunoprecipitation)/product/Amersham Pharmacia Biotech Ltd
Average 90 stars, based on 1 article reviews
mouse anti-p38-b2 (number 33–8700 used at 5 mg/immunoprecipitation) - by Bioz Stars, 2026-02
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human/mouse/rat p38 gamma antibody  (Bio-Techne corporation)
94
Bio-Techne corporation human/mouse/rat p38 gamma antibody
PI3K/AKT signaling pathway is involved in the inhibitory effect of RES on NF-κB activation. ( A ) GICs (412) were treated with DMSO (vehicle) and 5 μM, 10 μM, and 20 μM RES for 48 h. Total expression and phosphorylation of Akt and mTOR were analyzed by western blotting using anti-Akt, anti-p-Akt, anti-mTOR, anti-p-mTOR, antibodies. β-actin was used as a loading control; ( B ) Total expression and phosphorylation of JNK, ERK, and <t>p38</t> were analyzed by western blotting using anti-JNK, anti-p-JNK, anti-ERK, anti-p-ERK, anti-p38 anti-p-p38 antibodies. β-actin was used as a loading control; ( C ) GICs (412) were pre-treated with IGF-1 (200 ng·mL −1 ) or wortmannin (5 μM) for 30 min and then incubated in the presence or absence of RES (10 μM). GICs were then subjected to cell invasion assay. Then, randomly chosen fields were photographed (200×), and the number of cells that migrated to the lower surface was calculated as a percentage of invasion. Data are shown as the mean ± SD of three independent experiments by Student’s t -test. * p < 0.05, ** p < 0.01; ( D ) GICs were pre-treated with IGF-1 (200 ng·mL −1 ) or wormannin (5 μM) for 30 min and incubated in the presence or absence of RES (10 μM) for 48 h, and then, the cell lysates were subjected to western blotting with anti-p-Akt and anti-Akt antibodies. β-actin was used as a loading control. The CM were subjected to gelatin zymography assay to determine the activity of MMP-2.
Human/Mouse/Rat P38 Gamma Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human/mouse/rat p38 gamma antibody/product/Bio-Techne corporation
Average 94 stars, based on 1 article reviews
human/mouse/rat p38 gamma antibody - by Bioz Stars, 2026-02
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mouse monoclonal anti-synaptophysin p38  (Boehringer Ingelheim)
90
Boehringer Ingelheim mouse monoclonal anti-synaptophysin p38
PI3K/AKT signaling pathway is involved in the inhibitory effect of RES on NF-κB activation. ( A ) GICs (412) were treated with DMSO (vehicle) and 5 μM, 10 μM, and 20 μM RES for 48 h. Total expression and phosphorylation of Akt and mTOR were analyzed by western blotting using anti-Akt, anti-p-Akt, anti-mTOR, anti-p-mTOR, antibodies. β-actin was used as a loading control; ( B ) Total expression and phosphorylation of JNK, ERK, and <t>p38</t> were analyzed by western blotting using anti-JNK, anti-p-JNK, anti-ERK, anti-p-ERK, anti-p38 anti-p-p38 antibodies. β-actin was used as a loading control; ( C ) GICs (412) were pre-treated with IGF-1 (200 ng·mL −1 ) or wortmannin (5 μM) for 30 min and then incubated in the presence or absence of RES (10 μM). GICs were then subjected to cell invasion assay. Then, randomly chosen fields were photographed (200×), and the number of cells that migrated to the lower surface was calculated as a percentage of invasion. Data are shown as the mean ± SD of three independent experiments by Student’s t -test. * p < 0.05, ** p < 0.01; ( D ) GICs were pre-treated with IGF-1 (200 ng·mL −1 ) or wormannin (5 μM) for 30 min and incubated in the presence or absence of RES (10 μM) for 48 h, and then, the cell lysates were subjected to western blotting with anti-p-Akt and anti-Akt antibodies. β-actin was used as a loading control. The CM were subjected to gelatin zymography assay to determine the activity of MMP-2.
Mouse Monoclonal Anti Synaptophysin P38, supplied by Boehringer Ingelheim, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal anti-synaptophysin p38/product/Boehringer Ingelheim
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Image Search Results


PI3K/AKT signaling pathway is involved in the inhibitory effect of RES on NF-κB activation. ( A ) GICs (412) were treated with DMSO (vehicle) and 5 μM, 10 μM, and 20 μM RES for 48 h. Total expression and phosphorylation of Akt and mTOR were analyzed by western blotting using anti-Akt, anti-p-Akt, anti-mTOR, anti-p-mTOR, antibodies. β-actin was used as a loading control; ( B ) Total expression and phosphorylation of JNK, ERK, and p38 were analyzed by western blotting using anti-JNK, anti-p-JNK, anti-ERK, anti-p-ERK, anti-p38 anti-p-p38 antibodies. β-actin was used as a loading control; ( C ) GICs (412) were pre-treated with IGF-1 (200 ng·mL −1 ) or wortmannin (5 μM) for 30 min and then incubated in the presence or absence of RES (10 μM). GICs were then subjected to cell invasion assay. Then, randomly chosen fields were photographed (200×), and the number of cells that migrated to the lower surface was calculated as a percentage of invasion. Data are shown as the mean ± SD of three independent experiments by Student’s t -test. * p < 0.05, ** p < 0.01; ( D ) GICs were pre-treated with IGF-1 (200 ng·mL −1 ) or wormannin (5 μM) for 30 min and incubated in the presence or absence of RES (10 μM) for 48 h, and then, the cell lysates were subjected to western blotting with anti-p-Akt and anti-Akt antibodies. β-actin was used as a loading control. The CM were subjected to gelatin zymography assay to determine the activity of MMP-2.

Journal: Nutrients

Article Title: Resveratrol Inhibits the Invasion of Glioblastoma-Initiating Cells via Down-Regulation of the PI3K/Akt/NF-κB Signaling Pathway

doi: 10.3390/nu7064383

Figure Lengend Snippet: PI3K/AKT signaling pathway is involved in the inhibitory effect of RES on NF-κB activation. ( A ) GICs (412) were treated with DMSO (vehicle) and 5 μM, 10 μM, and 20 μM RES for 48 h. Total expression and phosphorylation of Akt and mTOR were analyzed by western blotting using anti-Akt, anti-p-Akt, anti-mTOR, anti-p-mTOR, antibodies. β-actin was used as a loading control; ( B ) Total expression and phosphorylation of JNK, ERK, and p38 were analyzed by western blotting using anti-JNK, anti-p-JNK, anti-ERK, anti-p-ERK, anti-p38 anti-p-p38 antibodies. β-actin was used as a loading control; ( C ) GICs (412) were pre-treated with IGF-1 (200 ng·mL −1 ) or wortmannin (5 μM) for 30 min and then incubated in the presence or absence of RES (10 μM). GICs were then subjected to cell invasion assay. Then, randomly chosen fields were photographed (200×), and the number of cells that migrated to the lower surface was calculated as a percentage of invasion. Data are shown as the mean ± SD of three independent experiments by Student’s t -test. * p < 0.05, ** p < 0.01; ( D ) GICs were pre-treated with IGF-1 (200 ng·mL −1 ) or wormannin (5 μM) for 30 min and incubated in the presence or absence of RES (10 μM) for 48 h, and then, the cell lysates were subjected to western blotting with anti-p-Akt and anti-Akt antibodies. β-actin was used as a loading control. The CM were subjected to gelatin zymography assay to determine the activity of MMP-2.

Article Snippet: The membrane was then blocked with 5% nonfat dry milk for 1 h at room temperature, and the protein levels were determined by overnight incubation with primary antibodies, which included antibodies specific for phospho-IKKα/β (H-470), Akt, phospho-Akt (Ser 473), IKKα/β, JNK, phospho-JNK (Thr183/185), IκBα, phospho-IκBα (Ser 32/36), p38, phospho-p38 (Thr 180), mmp-2, GFAP (Santa Cruz Biotechnology, USA), ERK1/2, phospho-ERK1/2 (Thr202/204), mTOR, phospho-mTOR (Ser 2448), NF-κB p65, Lamin A (Cell Signaling Technology, USA), Nestin (Abcam, Cambridge, UK), CD133 (Miltenyi Biotec, Bergisch Gladbach, Germany) and β-actin.

Techniques: Activation Assay, Expressing, Phospho-proteomics, Western Blot, Control, Incubation, Invasion Assay, Zymography Assay, Activity Assay